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alliance 4.7Gel documentation – An Introduction

A Gel Doc also known as a Gel Documentation system or Gel Image System or Gel Imager is equipment widely used in Molecular Biology Laboratories for the Imaging and Documentation of Nucleic Acid and Protein suspended within Polyacrylamide or Agarose Gels.

These Gels are typically Stained with Ethidium Bromide or other Fluoroscopes such as SYBR Green.

Generally a Gel Doc Includes,,,

an Ultraviolet (UV) Light Transformational,

a Hood to shield external light sources and protect the user from UV Exposure, and

a Camera for Image Capturing.

UV-transilluminatorElectrophoresis is an extremely common technique in molecular biology, used routinely to analyse DNA preparations in order to check quantity, size, structure and constitution of prepared samples. After separation on a gel (usually agarose), DNA is normally visualised under UV light after treatment with a fluorescent stain such as ethidium bromide. Electrophoresis is also used to study similar properties in protein samples though in this case, visualisation is normally under visible light after treatment with visible stains such as Coomassie or silver.

Whatever the sample or method of visualisation, it is often required or at least desirable to keep a record of the analysis for future reference and/or analysis. This can be achieved with instant photography to produce a “hard copy” print of the illuminated gel and its visible bands.  However, this method has been increasingly superseded by video documentation, which has two clear advantages:

  • Unlike with instant photography, a live video preview of the gel image can be seen prior to image “capture”.
  • The captured image can be saved as a computer file, which can be easily archived, printed when necessary and analysed, using suitable software.

UVIdocHD2In these days of routine, high throughput, analysis of DNA in particular, both of the above are enormously important to the efficiency of many research and analytical laboratories. The design of a gel documentation system should reflect not only the type of user and application, but also the number of users and the environment in which it is situated. The UVItec range therefore includes systems incorporating different levels of simplicity, portability, safety and robustness:

• FireReader V4 is preferable for the more frequent, dedicated user who requires precise control over the image characteristics (brightness, contrast, background level).
• UVIdoc HD5 is the most versatile system combining simplicity, safety and robustness, with a good level of  functionality. It is ideal for large, multi-user groups with many occasional users.
• UVIsave HD5 is preferable for users who require ultimate simplicity and portability and is ideal for small laboratories with limited budget and space.

uvitec transilluminatorsThe three video documentation systems UVIsave HD5UVIdoc HD5  and FireReader V4 are all capable of capturing images from blots, autoradiograms and colony plates, in addition to their primary function of image capture from fluorescent gels. All images captured are potentially analysable using the software provided with the systems, or with the optional UVIsoft analysis software packages.

Computer based systems
Platinum HD7

FireReader V4

 

Essential V4

 

 Highest performance gel doc systems available
• 2.8 megapixels
• Massive 16-bit pixel depth (65 536 grey levels)
• Protocol driven image acquisition
• USB connection
• Unrivalled sensitivity
• Dynamic range up to 4.8 orders of magnitude
• Patent UVI-PURE technology available
• Superb quality camera filter optimised for Ethidium Bromide
• Customise your own system with our wide variety of darkrooms & options
• Biofluorescence and multiplexing ready
 Massive 16-bit depth (65 536 grey levels) –
• 1.6 Megapixels extendable to 5.5 megapixels
• Scientific Sony chip CCD camera
• 9 wavelength illumination options
• Patented UVI-PURE technology available
• Auto-exposure and autofocus with motorised zoom option
• Extreme sensitivity for the faintest fluorescence sample
• Suitable for both routine documentation & critical quantitative applications
• Versatile and upgradeable
• USB connection
• “One touch” fully automated image acquisition program
• Wide variety of darkrooms and options to tailor your own system and fit your budget
 Scientific Sony chip CCD camera
• Ideal for publication and routine documentation
• 1.6 megapixel – 16-bit pixel depth
• Extreme ease of use
• Comprehensive range of models for any application or budget
• Ideal for a multi-user environment
• Good Laboratory Practice file
• Robust steel and stainless steel construction
• Wide variety of darkrooms and options to tailor your own system and fit your budget
Stand alone system
UVIdoc HD5
UVIsave HD5
• Cutting edge performance
• Stand-alone: no additional computer needed

• Ideal for multi-user environments
• Network capability
• 4 USB ports
• 1.4 megapixels
• Saturation monitoring of the live image
• 16-bit pixel depth

As simple as affordable
• Ideal for publication and routine imaging
• All the flexibility of an instant photography system
• Low cost documentation system with CDD scientific grade camera
• Stand-alone: no additional computer needed
• Ideal for multi-user environments
• Now with 8″ TFT grade LCD screen
• Print on a USB digital printer or save on a USB key
• Unlike digital camera systems, set your exposure time and monitor the saturation
• Free UVIdoc 1D software included for both image acquisition and analysis

Chemiluminescence documentation

Advanced systems
Alliance 7 series Unrivalled sensitivity
Alliance Chroma The multimodal imaging platform
Alliance LD The system which fit your budget
Alliance Mini All in one

Alliance 7 series Alliance Chroma
Ideal for resolution demanding applications such as 1D quantification, 2D gel, multiplexing,
biofluorescence and western blots
• No compromise: the best of dedicated chemi system and gel-doc gathered in one system
• Massive 16-bit performance
• Dynamic range up to 4.8 orders of magnitude
• Extreme 4.2 Megapixels resolution – 1.1 inch sensor
• Dynamic range up to 4.8 orders of magnitude
• Extremely bright fixed lens (f:0,95)
• Unique twin cooling system
• Patent pending UVI-PURE technology available
• “One touch” fully automated image acquisition program
• USB super-fast connection
• Ideal for resolution demanding applications such as 1D quantification, 2D gel, multiplexing,
biofluorescence and western blots
• No compromise: the best of dedicated chemi system and gel-doc gathered in one system
• Massive 16-bit performance
• Dynamic range up to 4.8 orders of magnitude
• Extreme 4.2 Megapixels resolution – 1.1 inch sensor
• Dynamic range up to 4.8 orders of magnitude
• Extremely bright fixed lens (f:0,95)
• Unique twin cooling system
• Patent pending UVI-PURE technology available
• “One touch” fully automated image acquisition program
• USB super-fast connection

Along with the above Uvitec also deals withhome page 2

  • TLC documentation
    UVIchromTLC imaging systems
  • Trans-illuminators
  • UV crosslinker
    UVIlink ultraviolet crosslinker.essential
  • UV lamps
    UVIliteultraviolet lamps
  • UV viewing cabinet
    UVIcabultraviolet viewing cabinets
  • UV radiometers
    UVImeterultraviolet radiometers

 UVIsoft Image quantification software

UVIband 1D analysis software
UVImap 1D analysis software
UVIbandmap 1D analysis software
UVIspot 2D analysis software

Special features and benefits

platinumIn designing UVIsoft analysis software, we have thought of everything. As a result, all UVIsoft analysis packages are:

User-Friendly: every aspect of UVIsoft is designed with ease of use in mind. This includes the very first step of opening an image, which is facilitated by the ability to choose the file from a “thumbnail” image gallery.

  • Powerful: all UVIsoft analysis  packages include the option of reliable, fully automatic background subtraction for band quantification and optional methods for correction of gel distortions during molecular weight calculations.
  • Flexible:Attractive as it may sound, “One Step” analysis places excessive reliance on automation and can detract from accurate gel or blot analysis. Analysis in UVIsoft packages is step-wise and at each analysis step, there is the facility for the user to check and (if necessary) edit the results of automatic analysis processes.
  • Versatile:In addition to the incredibly broad range of analysis functions available in UVIsoft, there are also functions to facilitate data presentation, reports and posters. The “Print Designer” function enables data tables plus images from all analysis fields to be automatically “pasted” into a presentation or report along with explanatory text. The report can then be saved as a template, so the same report can be prepared from other data.