Cecil Instruments - SPECTROSCOPY
Spectroscopy was originally the study of the interaction between radiation and matter as a function of wavelength ë. In fact, historically, spectroscopy referred to the use of visible light dispersed according to its wavelength, e.g. by a prism. Later the concept was expanded greatly to comprise any measurement of a quantity as function of either wavelength or frequency. Thus it also can refer to interactions with particle radiation or to a response to an alternating field or varying frequency í. An further extension of the scope of the definition added energy E as a variable, once the very close relationship E=hí for photons was realized. A plot of the response as a function of wavelength — or more commonly frequency — is referred to as a spectrum. Spectrometry is the measurement of these responses and an instrument which performs such measurements is a spectrometer
Many atoms emit or absorb visible light. In order to obtain a fine line spectrum, the atoms must be in a gas phase. This means that the substance has to be vaporized. The spectrum is studied in absorption or emission. Visible absorption spectroscopy is often combined with UV absorption spectroscopy in UV/Vis spectroscopy.
All atoms absorb in the Ultraviolet (UV) region because these photons are energetic enough to excite outer electrons. If the frequency is high enough, photo-ionization takes place. UV spectroscopy is also used in quantifying protein and DNA concentration as well as the ratio of protein to DNA concentration in a solution. Several amino acids usually found in protein, such as tryptophan, absorb light in the 280nm range and DNA absorbs light in the 260nm range. For this reason, the ratio of 260/280nm absorbance is a good general indicator of the relative purity of a solution in terms of these two macromolecules. Reasonable estimates of protein or DNA concentration can also be made this way using Beer’s law.
Various Models Include
Super Aurius Model: CE3021 : Wavelength range of 190-1000nm with 1.8nm Bandpass
- Performance with convenience
- Absorbance Accuracy
- Sixthscreen width viewing
- Ease of Operation
- Accurate Quantification
- Wavelength Scanning
Download Super Aurius Date sheet for More Info
CE7400: <2nm pharma compliant optical band pass with wavelength range of 190-1100nm
CE7400S: 1nm optical band pass with wavelength range of 190-1100nm
CE7500: 0.5, 1, 2 and 4nm variable band pass with wavelength range of 190-1100nm
CE7200: <2nm optical band pass with wavelength range of 190-900nm
- Variable or Fixed Bandwidth
- Double beam optical System
- Scans viewed by scrolling
- Scan storage
- Multi User convenience
- Reflectance and Gel Scanning
- Data Stream
- PC data Manipulation
- Band Quantification
CE9200: 1.8nm Optical Band pass with wavelength range of 190-800nm
CE9500: Variable optical Band pass 0.5, 1, 2 and 4nm with wavelength range of 190-900nm
- Variable optical band width
- Top Performance optical System
- Turbid Sampling Optics
- Integral Data Storage
- Assay Programming
- Dynamic 8scan recall
- Method Stoarge-100
- Spectral Storage – 100
- Curve Storage – 100
- Code Protected storage
- Validation Software
Other Dedicated Application equipment
AquaQuest – 190-1000nm with 4nm Bandwidth used for Water Analysis
BioQuest – 190-1000nm with 4nm Bandwidth used for Bio Analysis, Scanning
BioAquarius – 190-900 nm with 1.8nm Band width used for fully programmed Bioanalysis
Dietquest - 190-1000nm with 18.nm
325-1000nm with 4nm
190-900nm with 1.8nm used for Food & Beverage Analysis.
GenQuest - 190-370, 595 & 600 nm with 4nm Band Width used for Molecular Biology
PharmaQuest : 190-800nm with 1.8nm Bandwidth used for Dissolution Teste
Cecil Instruments - Reflectscan
Universal Reflectance Measurements
The ReflectaScan Reflectance Spectrophotometer is designed as a universal reflectance measuring instrument capable of scanning samples over the wavelength range 190-1000nm. An integral printer plots results and an external dot matrix or colour inkjet printer may be used if required.
Diffuse Reflectance Measurements
The integrating sphere system allows a range of measurements to be made.
* Diffuse and specular samples
* Total hemispherical reflectance
* Specular excluded diffuse reflectance
* Transmittance of turbid or scattering samples
* Diffuse reflectance of powder samples
The compact sphere, which mounts within the instrument's sample compartment, is made from SpectralonTM which has the highest reflectance over the UV and visible region of any known substance. This gives high energy throughput resulting in excellent performance. The design is versatile, easy to use and allows for the elimination of substitution errors.
A 0° sample rest enables measurements to be carried out with the specular component eliminated. Substituted by a 11° sample rest, total hemispherical reflectance is measured.
A dedicated high performance SpectralonTM integrating sphere is used for diffuse reflectance and diffuse transmittance measurements. The sphere unit has its own silicon diode detector which uses a separate signal channel within the Spectrophotometer.
Turbid or Scattering Samples
The sphere unit is equipped with a 10mm path length cuvette holder so that transmittance measurements may be made for both turbid or normal samples. Scattering films may also be accommodated for measurement.
A dedicated specular reflectance unit is used for specular reflectance measurements of surfaces and coated ophthalmic lenses. The unit is ideal for quality assurance in the production of anti-reflection coated ophthalmic lenses, many of these systems are now in use around the world.
Diffuse Reflectance of Samples
A scan is shown here of a poppy coloured paint sample from the Dulux paint range and also an overlay of scans for two blue paint samples each specified by its British Standard number shown on the chart.
Below is the reflectance scan of a plain white copy paper which shows a good flat response over the visible region, with fluorescence stimulated by UV radiation showing in the short wavelength region, but the origin of which is of longer wavelength.
CE3055- Reflectscan – Reflectscan Spectrophotometer with 4nm Band pass, 190-1100nm wavelength range
- Diffuse Reflectance Measurements
- Universal Reflectance Measurements
- Integrating Sphere
- Colour Computation using Diffuse Reflectance
- Turbid or Scattering Sample
- Coated lens Reflectance
- Specula Reflectance
- Ophthalmic Lens Transmission
- Automatic Thin film measurement
- Automation Colour computations